Keeping in view the clinical importance of leptospirosis haematobiochemical and urine analysis were carried out on 500

(cattle-398, buffalo-102) blood/serum and 304 (cattle-232, buffalo-72) urine samples of both seropositive (cattle-51, buffalo-16) and seronegative (cattle-347, buffalo-86) animals from different district of South Gujarat region. A signifi cant decrease in the values of PCV, MCH and MCHC was noted in seropositive group of cattle in comparison to seronegative group. Such difference among seropositive and seronegative groups could not be recorded in buffaloes. The mean values of ALT, AST and bilirubin registered an increase at signifi cant level in seropositive cattle in comparison to seronegative. Among buffaloes, the mean values of ALT increased and total protein decreased signifi cantly (P <0.05) in seropositive buffaloes in comparison to seronegative buffaloes. On urine analyses (n=304; cattle=232, buffaloes=72) hardly any signifi cant difference was noted in various parameter studied in either species in seropositive and seronegative animals. All the urine samples (304) collected were subjected to Dark Field Microscopy (DFM) proved to be negative for leptospires